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Recombinant human osteopontin expressed in Nicotiana benthamiana stimulates osteogenesis related genes in human periodontal ligament cells


Abstract Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography. Structural analysis indicated that plant-produced hOPN had a structure similar to commercial HEK cell-produced hOPN. Biological function o... (more)
Created Date 2017-12-11
Contributor Rattanapisit, Kaewta (Author) / Abdulheem, Supaniga (Author) / Chaikeawkaew, Daneeya (Author) / Kubera, Anchanee (Author) / Mason, Hugh (ASU author) / Ma, Julian K-C (Author) / Pavasant, Prasit (Author) / Phoolcharoen, Waranyoo (Author) / Biodesign Institute / Immunotherapy, Vaccines and Virotherapy
Series SCIENTIFIC REPORTS
Type Text
Extent 9 pages
Language English
Identifier DOI: 10.1038/s41598-017-17666-7 / ISSN: 2045-2322
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Citation Rattanapisit, K., Abdulheem, S., Chaikeawkaew, D., Kubera, A., Mason, H. S., Ma, J. K., . . . Phoolcharoen, W. (2017). Recombinant human osteopontin expressed in Nicotiana benthamiana stimulates osteogenesis related genes in human periodontal ligament cells. Scientific Reports, 7(1). doi:10.1038/s41598-017-17666-7
Note The final version of this article, as published in Scientific Reports, can be viewed online at: http://www.nature.com/articles/s41598-017-17666-7
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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