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Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes


Abstract Background
Virus infections often result in quasispecies of viral strains that can have dramatic impacts on disease outcomes. However, sequencing of viruses to determine strain composition is time consuming and often cost-prohibitive. Rapid, cost-effective methods are needed for accurate measurement of virus diversity to understand virus evolution and can be useful for experimental systems.
Methods
We have developed a novel molecular method for sequence-specific detection of RNA virus genetic variants called Tentacle Probes. The probes are modified molecular beacons that have dramatically improved false positive rates and specificity in routine qPCR. To validate this approach, we have designed Tentacle Probes for two different strains of Ly... (more)
Created Date 2017-10-13
Contributor Franco Achury, Lina (ASU author) / Holechek, Susan (ASU author) / Caplan, Michael (ASU author) / Blattman, Joseph (ASU author) / College of Liberal Arts and Sciences / School of Molecular Sciences / School of Life Sciences / School of Human Evolution and Social Change / Simon M. Levin Mathematical, Computational and Modeling Sciences Center / Ira A. Fulton Schools of Engineering / School of Biomedical and Health Systems Engineering / Biodesign Institute / Immunotherapy, Vaccines and Virotherapy
Series VIROLOGY JOURNAL
Type Text
Extent 11 pages
Language English
Identifier DOI: 10.1186/s12985-017-0863-9 / ISSN: 1743-422X
Copyright
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Citation Franco, L. S., Holechek, S. A., Caplan, M. R., & Blattman, J. N. (2017). Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes. Virology Journal, 14(1). doi:10.1186/s12985-017-0863-9
Note The electronic version of this article is the complete one and can be found online at: https://virologyj.biomedcentral.com/articles/10.1186/s12985-017-0863-9
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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