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Large-Scale Kinetic Analyses of Protein-Protein Interactions: Advancing the Understanding of Post Translational Modifications in Biological Regulation


Abstract Signal transduction networks comprising protein-protein interactions (PPIs) mediate homeostatic, diseased, and therapeutic cellular responses. Mapping these networks has primarily focused on identifying interactors, but less is known about the interaction affinity, rates of interaction or their regulation. To better understand the extent of the annotated human interactome, I first examined > 2500 protein interactions within the B cell receptor (BCR) signaling pathway using a current, cutting-edge bioluminescence-based platform called “NanoBRET” that is capable of analyzing transient and stable interactions in high throughput. Eighty-three percent (83%) of the detected interactions have not been previously reported, indicating that much o... (more)
Created Date 2018
Contributor Petritis, Brianne Ogata (Author) / LaBaer, Joshua (Advisor) / Lake, Douglas (Committee member) / Wang, Shaopeng (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Molecular biology / Physical chemistry / kinetics / NAPPA / protein microarray / protein-protein interactions / SPRi / surface plasmon resonance
Type Doctoral Dissertation
Extent 454 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Biological Design 2018
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis