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Life In Motion: Visualizing Biomacromolecules By Time-Resolved Serial Femtosecond Crystallography

Abstract Time-resolved serial femtosecond crystallography is an emerging method that allows for structural discovery to be performed on biomacromolecules during their dynamic trajectory through a reaction pathway after activation. This is performed by triggering a reaction on an ensemble of molecules in nano- or microcrystals and then using femtosecond X-ray laser pulses produced by an X-ray free electron laser to collect near-instantaneous data on the crystal. A full data set can be collected by merging a sufficient number of these patterns together and multiple data sets can be collected at different points along the reaction pathway by manipulating the delay time between reaction initiation and the probing X-rays. In this way, these ‘snapshot’... (more)
Created Date 2018
Contributor Coe, Jesse (Author) / Fromme, Petra (Advisor) / Sayres, Scott (Advisor) / Mujica, Vladimiro (Committee member) / Redding, Kevin (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Physics / Biochemistry / crystallography / Photosystem / SFX / time-resolved / XFEL
Type Doctoral Dissertation
Extent 302 pages
Language English
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Chemistry 2018
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis