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Electrochemical Investigation of Electron Bifurcating Oxidoreductases


Abstract Biological systems have long been known to utilize two processes for energy conservation: substrate-level phosphorylation and electron transport phosphorylation. Recently, a new bioenergetic process was discovered that increases ATP yields: flavin-based electron bifurcation (FBEB). This process couples an energetically favorable reaction with an energetically unfavorable one to conserve energy in the organism. Currently, the mechanisms of enzymes that perform FBEB are unknown. In this work, NADH-dependent reduced ferredoxin:NADP+ oxidoreductase (Nfn), a FBEB enzyme, is used as a model system to study this phenomenon. Nfn is a heterodimeric enzyme that reversibly couples the exergonic reduction of NADP+ by reduced ferredoxin with the endergo... (more)
Created Date 2018
Contributor Jennings, David (Author) / Jones, Anne K (Advisor) / Redding, Kevin E (Committee member) / Torres, Cesar I (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Biochemistry
Type Doctoral Dissertation
Extent 196 pages
Language English
Copyright
Note Doctoral Dissertation Chemistry 2018
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis