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Reconstitution of the Heliobacterial Reaction Center Into Proteoliposomes and Restoration of Its Interaction with Membrane-bound Cytochrome c553


Abstract To mimic the membrane environment for the photosynthetic reaction center of the photoheterotrophic Heliobacterium modesticaldum, a proteoliposome system was developed using the lipids found in native membranes, as well as a lipid possessing a Ni(II)-NTA head group. The liposomes were also saturated with menaquinone-9 to provide further native conditions, given that menaquinone is active within the heliobacterial reaction center in some way. Purified heliobacterial reaction center was reconstituted into the liposomes and a recombinant cytochrome c553 was decorated onto the liposome surface. The native lipid-attachment sequence of cytochrome c553 was truncated and replaced with a hexahistidine tag. Thus, the membrane-anchoring observed in viv... (more)
Created Date 2018
Contributor Johnson, William Alexander (Author) / Redding, Kevin E (Advisor) / Van Horn, Wade D (Committee member) / Jones, Anne K (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Bioenergetics / Electron Transfer / Heliobacteria / Liposomes / Photosynthesis
Type Masters Thesis
Extent 33 pages
Language English
Copyright
Note Masters Thesis Chemistry 2018
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis