Label Free Methods for the Quantification of Molecular Interaction with Membrane Protein on Cell Surface
|Abstract||Measuring molecular interaction with membrane proteins is critical for understanding cellular functions, validating biomarkers and screening drugs. Despite the importance, developing such a capability has been a difficult challenge, especially for small molecules binding to membrane proteins in their native cellular environment. The current mainstream practice is to isolate membrane proteins from the cell membranes, which is difficult and often lead to the loss of their native structures and functions. In this thesis, novel detection methods for in situ quantification of molecular interactions with membrane proteins are described.
First, a label-free surface plasmon resonance imaging (SPRi) platform is developed for the in situ detection o... (more)
|Contributor||Zhang, Fenni (Author) / Tao, Nongjian (Advisor) / Chae, Junseok (Committee member) / Borges, Chad (Committee member) / Jing, Tianwei (Committee member) / Wang, Shaopeng (Committee member) / Arizona State University (Publisher)|
|Subject||Electrical engineering / Biochemistry / Biomedical engineering / Label-free / Membrane protein / Molecular interaction / Optical imaging / Single cell / Vesicle release|
|Note||Doctoral Dissertation Electrical Engineering 2018|
|Collaborating Institutions||Graduate College / ASU Library|
|Additional Formats||MODS / OAI Dublin Core / RIS|