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Label Free Methods for the Quantification of Molecular Interaction with Membrane Protein on Cell Surface


Abstract Measuring molecular interaction with membrane proteins is critical for understanding cellular functions, validating biomarkers and screening drugs. Despite the importance, developing such a capability has been a difficult challenge, especially for small molecules binding to membrane proteins in their native cellular environment. The current mainstream practice is to isolate membrane proteins from the cell membranes, which is difficult and often lead to the loss of their native structures and functions. In this thesis, novel detection methods for in situ quantification of molecular interactions with membrane proteins are described.

First, a label-free surface plasmon resonance imaging (SPRi) platform is developed for the in situ detection o... (more)
Created Date 2018
Contributor Zhang, Fenni (Author) / Tao, Nongjian (Advisor) / Chae, Junseok (Committee member) / Borges, Chad (Committee member) / Jing, Tianwei (Committee member) / Wang, Shaopeng (Committee member) / Arizona State University (Publisher)
Subject Electrical engineering / Biochemistry / Biomedical engineering / Label-free / Membrane protein / Molecular interaction / Optical imaging / Single cell / Vesicle release
Type Doctoral Dissertation
Extent 143 pages
Language English
Copyright
Note Doctoral Dissertation Electrical Engineering 2018
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis