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Opening Chromatin and Improving CRISPR / Cas9 Editing


Abstract The research question explored in this thesis is how CRISPR mediated editing is influenced by artificially opened chromatin in cells. Closed chromatin poses a barrier to Cas9 binding and editing at target genes. Synthetic pioneer factors (PFs) are a promising new approach to artificially open condensed heterochromatin allowing greater access of target DNA to Cas9. The Haynes lab has constructed fusions of enzymatic chromatin-modifying domains designed to remodel chromatin and increase Cas9 editing efficiency. With a library of PFs available, this research focuses on analyzing the behavior of Cas9 in chromatin that has been artificially opened by PFs. The types and frequency of INDELs (insertions & deletions) were determined after non-ho... (more)
Created Date 2019
Contributor Hamna, Syeda Fatima (Author) / Haynes, Karmella A (Advisor) / Stabenfeldt, Sarah (Advisor) / Tian, Xiaojun (Committee member) / Arizona State University (Publisher)
Subject Bioengineering / Biomedical engineering / chromatin engineering / CRISPR / Cas9 editing / synthetic pioneer factors
Type Masters Thesis
Extent 55 pages
Language English
Copyright
Note Masters Thesis Biomedical Engineering 2019
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis