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Genetic and Biochemical Insights into the Mycobacterial PrrAB System as a Regulator of Respiration and Central Metabolism


Abstract Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is the 10th leading cause of death, worldwide. The prevalence of drug-resistant clinical isolates and the paucity of newly-approved antituberculosis drugs impedes the successful eradication of Mtb. Bacteria commonly use two-component systems (TCS) to sense their environment and genetically modulate adaptive responses. The prrAB TCS is essential in Mtb, thus representing an auspicious drug target; however, the inability to generate an Mtb ΔprrAB mutant complicates investigating how this TCS contributes to pathogenesis. Mycobacterium smegmatis, a commonly used M. tuberculosis genetic surrogate was used here. This work shows that prrAB is not essential in M. smegmatis. Duri... (more)
Created Date 2019
Contributor Maarsingh, Jason (Author) / Haydel, Shelley E (Advisor) / Roland, Kenneth (Committee member) / Sandrin, Todd (Committee member) / Bean, Heather (Committee member) / Arizona State University (Publisher)
Subject Microbiology / metabolism / Mycobacterium smegmatis / Mycobacterium tuberculosis / PrrAB / respiration / two-component systems
Type Doctoral Dissertation
Extent 175 pages
Language English
Copyright
Note Doctoral Dissertation Microbiology 2019
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis