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RNA-Based Computing Devices for Intracellular and Diagnostic Applications

Abstract The fundamental building blocks for constructing complex synthetic gene networks are effective biological parts with wide dynamic range, low crosstalk, and modularity. RNA-based components are promising sources of such parts since they can provide regulation at the level of transcription and translation and their predictable base pairing properties enable large libraries to be generated through in silico design. This dissertation studies two different approaches for initiating interactions between RNA molecules to implement RNA-based components that achieve translational regulation. First, single-stranded domains known as toeholds were employed for detection of the highly prevalent foodborne pathogen norovirus. Toehold switch riboregulators... (more)
Created Date 2019
Contributor MA, DUO (Author) / Green, Alexander (Advisor) / Mangone, Marco (Committee member) / Liu, Yan (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Cell-free system / logic gates / Paper-based platform / Riboregulator / RNA-based genetic circuits / Synthetic biology
Type Doctoral Dissertation
Extent 105 pages
Language English
Note Doctoral Dissertation Biochemistry 2019
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis