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Controlled Epigenetic Silencing and Tandem Histone-Binding Transcriptional Activation


Abstract Fusion proteins that specifically interact with biochemical marks on chromosomes represent a new class of synthetic transcriptional regulators that decode cell state information rather than deoxyribose nucleic acid (DNA) sequences. In multicellular organisms, information relevant to cell state, tissue identity, and oncogenesis is often encoded as biochemical modifications of histones, which are bound to DNA in eukaryotic nuclei and regulate gene expression states. In 2011, Haynes et al. showed that a synthetic regulator called the Polycomb chromatin Transcription Factor (PcTF), a fusion protein that binds methylated histones, reactivated an artificially-silenced luciferase reporter gene. These synthetic transcription activators are derived ... (more)
Created Date 2019
Contributor Vargas, Daniel A. (Author) / Haynes, Karmella (Advisor) / Wang, Xiao (Committee member) / Mills, Jeremy (Committee member) / Arizona State University (Publisher)
Subject Biomedical engineering / Bioinformatics / Biology / PcTF / Polycomb / Reactivation / Synthetic biology / Transcriptional activation / Vargas
Type Masters Thesis
Extent 58 pages
Language English
Copyright
Note Masters Thesis Biological Design 2019
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis