Sample Delivery Enabled by 3D Printing for Reduced Sample Consumption and Mix-and-Inject Serial Crystallography at X-ray Free Electron Lasers
|Abstract||Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) has enabled the determination of damage-free protein structures at ambient temperatures and of reaction intermediate species with time resolution on the order of hundreds of femtoseconds. However, currently available XFEL facility X-ray pulse structures waste the majority of continuously injected crystal sample, requiring a large quantity (up to grams) of crystal sample to solve a protein structure. Furthermore, mix-and-inject serial crystallography (MISC) at XFEL facilities requires fast mixing for short (millisecond) reaction time points (𝑡"), and current sample delivery methods have complex fabrication and assembly requirements.
To reduce sample consum... (more)
|Contributor||Echelmeier, Austin (Author) / Ros, Alexandra (Advisor) / Levitus, Marcia (Committee member) / Weierstall, Uwe (Committee member) / Arizona State University (Publisher)|
|Subject||Chemistry / Fluid mechanics / 3D Printing / Droplets / Microfluidics / Micromixer / Serial Femtosecond Crystallography / X-ray Free Electron Laser|
|Note||Doctoral Dissertation Chemistry 2019|
|Collaborating Institutions||Graduate College / ASU Library|
|Additional Formats||MODS / OAI Dublin Core / RIS|