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Sample Delivery Enabled by 3D Printing for Reduced Sample Consumption and Mix-and-Inject Serial Crystallography at X-ray Free Electron Lasers


Abstract Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) has enabled the determination of damage-free protein structures at ambient temperatures and of reaction intermediate species with time resolution on the order of hundreds of femtoseconds. However, currently available XFEL facility X-ray pulse structures waste the majority of continuously injected crystal sample, requiring a large quantity (up to grams) of crystal sample to solve a protein structure. Furthermore, mix-and-inject serial crystallography (MISC) at XFEL facilities requires fast mixing for short (millisecond) reaction time points (𝑡"), and current sample delivery methods have complex fabrication and assembly requirements.

To reduce sample consum... (more)
Created Date 2019
Contributor Echelmeier, Austin (Author) / Ros, Alexandra (Advisor) / Levitus, Marcia (Committee member) / Weierstall, Uwe (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Fluid mechanics / 3D Printing / Droplets / Microfluidics / Micromixer / Serial Femtosecond Crystallography / X-ray Free Electron Laser
Type Doctoral Dissertation
Extent 211 pages
Language English
Copyright
Note Doctoral Dissertation Chemistry 2019
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


  Full Text
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Description Dissertation/Thesis
  Supplementary Video D1.avi
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Description Supplementary Video D.1 - Droplet formation in a 3D printed droplet generator