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Exploring the Telomeric Repeat Addition Processivity of Vertebrate Telomerase


Abstract Telomerase is a special reverse transcriptase that extends the linear chromosome termini in eukaryotes. Telomerase is also a unique ribonucleoprotein complex which is composed of the protein component called Telomerase Reverse Transcriptase (TERT) and a telomerase RNA component (TR). The enzyme from most vertebrate species is able to utilize a short template sequence within TR to synthesize a long stretch of telomeric DNA, an ability termed "repeat addition processivity". By using human telomerase reconstituted both in vitro (Rabbit Reticulocyte Lysate) and in vivo (293FT cells), I have demonstrated that a conserved motif in the reverse transcriptase domain of the telomerase protein is crucial for telomerase repeat addition proces... (more)
Created Date 2010
Contributor Xie, Mingyi (Author) / Chen, Julian J.L. (Advisor) / Yan, Hao (Committee member) / Wachter, Rebekka M. (Committee member) / Arizona State University (Publisher)
Subject Chemistry, Biochemistry / Biology, Molecular / biochemistry / molecular biology / motif 3 / repeat addition processivity / reverse transcriptase / RNA/DNA duplex
Type Doctoral Dissertation
Extent 222 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Biochemistry 2010
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis