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Exploring peptide space for enzyme modulators


Abstract Enzymes which regulate the metabolic reactions for sustaining all living things, are the engines of life. The discovery of molecules that are able to control enzyme activity is of great interest for therapeutics and the biocatalysis industry. Peptides are promising enzyme modulators due to their large chemical diversity and the existence of well-established methods for library synthesis. Microarrays represent a powerful tool for screening thousands of molecules, on a small chip, for candidates that interact with enzymes and modulate their functions. In this work, a method is presented for screening high-density arrays to discover peptides that bind and modulate enzyme activity. A viscous polyvinyl alcohol (PVA) solution was applied to array... (more)
Created Date 2010
Contributor Fu, Jinglin (Author) / Woodbury, Neal W (Advisor) / Johnston, Stephen A (Committee member) / Ghirlanda, Giovanna (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Analytical Chemistry / Bioinformatics / Biocatalysis / Enzyme / Enzyme immobilization / Enzyme Modulation / Microarray / Peptide
Type Doctoral Dissertation
Extent 180 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Chemistry 2010
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis