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Synechocystis Mutants Lacking Genes Potentially Involved in Carotenoid Metabolism


Abstract Like most other phototrophic organisms the cyanobacterium Synechocystis sp. PCC 6803 produces carotenoids. These pigments often bind to proteins and assume various functions in light harvesting, protection from reactive oxygen species (ROS) and protein stabilization. One hypothesis was that carotenoids bind to the surface (S-)layer protein. In this work the Synechocystis S-layer protein was identified as Sll1951 and the effect on the carotenoid composition of this prokaryote by disruption of sll1951 was studied. Loss of the S-layer, which was demonstrated by electron microscopy, did not result in loss of carotenoids or changes in the carotenoid profile of the mutant, which was shown by HPLC and protein analysis. Although Δsll1951 w... (more)
Created Date 2011
Contributor Trautner, Christoph (Author) / Vermaas, Willem Fj (Advisor) / Chandler, Douglas E (Committee member) / Misra, Rajeev (Committee member) / Bingham, Scott E (Committee member) / Arizona State University (Publisher)
Subject Microbiology / Molecular Biology / Biochemistry / carotenoids / cyanobacteria / labeling / S-layer / synechocystis
Type Doctoral Dissertation
Extent 159 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Microbiology 2011
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis