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Femtosecond X-ray Nanocrystallography of Membrane Proteins


Abstract Membrane proteins are very important for all living cells, being involved in respiration, photosynthesis, cellular uptake and signal transduction, amongst other vital functions. However, less than 300 unique membrane protein structures have been determined to date, often due to difficulties associated with the growth of sufficiently large and well-ordered crystals. This work has been focused on showing the first proof of concept for using membrane protein nanocrystals and microcrystals for high-resolution structure determination. Upon determining that crystals of the membrane protein Photosystem I, which is the largest and most complex membrane protein crystallized to date, exist with only a hundred unit cells with sizes of less than 200 nm... (more)
Created Date 2011
Contributor Hunter, Mark S. (Author) / Fromme, Petra (Advisor) / Wolf, George (Committee member) / Levitus, Marcia (Committee member) / Arizona State University (Publisher)
Subject Biophysics / crystallography / femtosecond / membrane protein / microcrystals / structural biology / XFEL
Type Doctoral Dissertation
Extent 290 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Chemistry 2011
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis